Multiplex real-time PCR for the detection and quantification of DNA from four transgenic soy Mon89788, A5547-127, Roundup Ready, A2704-12 and lectin

摘要

In the past, the transgenic soybean Roundup Ready was the predominantly cultivated transgenic soybean. With the announcement of Monsanto Corp. that this trait will be replaced by Mon89788 and the release of other company';s transgenic soy crops, this unique status of one trait has ended. Therefore, a multiplex quantitative real-time PCR system was developed and characterized for three additional transgenic traits Mon89788, A2704-12 and A5547-127. It showed amplification efficiency, correlation and sensitivity similar to the single PCR systems applied therein. This system allows relative multiplex quantification and/or delta-delta Ct method and therefore an efficient control of products during production, trade and sale.

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