NGS-based accurate and efficient detection of circulating cell-free mitochondrial DNA in cancer patients

摘要

Mitochondrial DNA mutations are closely implicated in the pathogenesis of multiple cancers, making circulating cell-free mitochondrial DNA (ccf-mtDNA) a potential non-invasive tumor biomarker. However, an effective approach to comprehensively profile ccf-mtDNA mutations is still lacking. Here, we first characterized ccf-mtDNA by low-depth whole genome sequencing (WGS) and found that plasma DNA samples exhibited a dramatic decrease in mtDNA copy number when compared with fresh tumor tissues. Further analysis revealed that plasma ccf-mtDNA had a biased distribution of fragment size with peak around 90 bp. Based on these insights, we developed a robust captured-based mtDNA deep sequencing approach that enable accurate and efficient detection of plasma ccf-mtDNA mutations by systematic optimization of probe quantity and length, hybridization temperature, and PCR amplification cycles. Moreover, we found that placement of isolated plasma for 6 hours at both 4°C and RT led to dramatic decrease of ccf-mtDNA stability, highlighting the importance of proper plasma sample processing. We further showed that the optimized approach can successfully detect a substantial fraction of tumor-specific mtDNA mutations in plasma ccf-mtDNA specifically from hepatocellular carcinoma (HCC) patients but not from colorectal cancer (CRC) patients, suggesting the presence of a potential cancer-specific difference in the abundance of tumor-derived mtDNA in plasma. Graphical Download : Download high-res image (279KB)Download : Download full-size image

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